Trypsin Inhibitor from Soybean Whey Wastewater: Isolation, Purification and Stability

Soybean trypsin inhibitor (STI) was obtained from simulated soybean whey wastewater through a sustainable method consisting of isoelectric precipitation, ammonium sulfate salting out, and gel filtration chromatography, the effect temperature, pH, pepsin on stability STI also discussed. The results showed that recovery rate inhibitory activity 89.47%, purity specific were 71.11%, 1442.5 TIU/mg in conditions pH 4.0 40% saturation. Kunitz (KTI) Bowman–Brik (BBI) via their levels 1733.5 2588.3 TIU/mg, respectively. displayed good over wide temperature range. STI, KTI, BBI all resistant to hydrolysis, ability ranked as > KTI. These findings will provide theoretical basis for recycling promoting better active compound utilization.